How to Construct a cDNA Library

This article describes how to construct a cDNA library from eukaryotic cells. cDNA libraries are a valuable tool for studying gene expression, as they contain all of the expressed genes in a cell, but not the introns or other non-coding regions.

Here are the steps involved in constructing a cDNA library:

1. Isolate mRNA from the cells of interest.
2. Reverse transcribe the mRNA into cDNA.
3. Clone the cDNA into a vector, such as a plasmid or a bacterial artificial chromosome (BAC).
4. Screen the library for clones that contain the genes of interest.

CDNA libraries are a powerful tool for studying gene expression. They can be used to identify genes that are involved in specific biological processes, to study the regulation of gene expression, and to clone genes for further study.